FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 99-101.doi: 10.7506/spkx1002-6300-200918018

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Extraction and Purification of Polysaccharides from Herba Moslae

YANG Chao,LI Jing-en,QIU Zeng-hui,NIE Shao-ping*,YIN Jun-yi,LI Chang,XIE Ming-yong   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2009-04-28 Online:2009-09-15 Published:2010-12-29
  • Contact: NIE Shao-ping E-mail:spnie@ncu.edu.cn

Abstract:

Crude water-soluble polysaccharides were isolated from Herba Moslae by hot water extraction and ethanol precipitation. After protein and pigment removal, the refined polysaccharides were separated by DEAE-52 cellulose anionexchange chromatography, and two fractions Ⅰand Ⅱ were obtained. The main fraction Ⅱ was further purified by Sephacryl S-300 gel chromatography and a polysaccharide named HMPⅡ was obtained. The purity of HMPⅡ was determined to be 93.55% by high performance gel permeation chromatography (HPGPC).

Key words: polysaccharides from Herba Moslae, extraction, purification

CLC Number: